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Expression of nm23
and KAI1 and their clinical significance in gallbladder adenocarcinoma.
Zhonghua Zhong Liu Za Zhi. 2008 Jun;30(6):441-3.
OBJECTIVE: To
investigate the expression of two tumor metastasis suppressor genes
nm23 and KAI1 in gallbladder adenocarcinoma, and their
clinicopathological significance. METHODS: Specimens and clinical data
from 31 gallbladder adenocarcinoma patients were collected.
Histopathological grading and the expression of nm23 and KAI1 were
detected by HE and immunohistochemical staining, respectively. All
cases were followed up for at least three years. RESULTS:
Immunohistochemical staining showed that the positive rate of nm23 and
KAI1 proteins was 71.0% (22/31) and 61.3% (19/31), respectively. The
positive expression rates of nm23 and KAI1 proteins in the early stage
carcinomas were significantly higher than those in the moderate and
advanced stage ones (P exact = 0.0051 and P exact = 0.0084), and both
had an negative correlation with clinicopathologic stage (P trend =
0.0047 and P trend = 0.0058). There was a significant difference in
the expression of nm23 and KAI1 proteins among well, moderately and
poorly differentiated carcinomas (P exact = 0.0328 and P exact =
0.0020). The expression of nm23 and KAI1 was positively correlated
with histopathological grade (P trend = 0.0086 and P trend = 0.0006).
There was also a significant difference in the expression of nm23 and
KAI1 proteins between 17 survival and 14 dead patients (P exact =
0.0038 and P exact = 0.0001 ). A synergistic effect of nm23 and KAI1
protein on the survival was observed , and seemed to be more important
than any individual gene alone (P exact = 0.0005). CONCLUSION: The
expressions of nm23 and KAI1 proteins are negatively correlated with
clinical stage, but positively with histopathological grade in
gallbladder adenocarcinoma. These two tumor metastasis suppressor
genes may act synergistically to inhibit the tumor metastasis.
Clinicopathologic features of gallbladder adenocarcinoma with marked
stromal fibrosis--a report of 19 cases.
Ai Zheng.
2006 Jul;25(7):896-900.
BACKGROUND &
OBJECTIVE: Macropathologic types of gallbladder cancer are mostly
polyp type, intumescent type, and cauliflower form lump. Its
histological types include well or poorly differentiated
adenocarcinoma, mucinous adenocarcinoma, and undifferentiated cancer.
This research was to explore the clinicopathologic features of
gallbladder adenocarcinoma with marked stromal fibrosis. METHODS:
Pathology of 19 cases of gallbladder adenocarcinoma with marked
stromal fibrosis was observed using a light microscopy and SP
immunohistochemistry. Clinicopathologic features of 19 patients were
analyzed. RESULTS: Most of the patients had long-term history of
cholecystitis gallbladder calculus. B ultrasound showed that the
gallbladder wall was irregularly thickened or presented nodosity.
Observed with naked eyes, gallbladder adenocarcinoma with marked
stromal fibrosis did not form cancer nodule and extrude into the
gallbladder lumen, the gallbladder wall showed regional thickening, a
few cases showed diffuse irregular thickening. Observed under a light
microscope, the adenocarcinoma cells were mostly arranged as single
layers, seldom arranged as multiple layers, and formed adenoid
structures with different sizes, various shapes, and irregular
arrangement; the nuclei were heterogenic with haryomitosis presented
in a few cases; inflammatory cells were infiltrated in hyperplastic
fibrous connective tissue of some cases. According to immune
phenotyping, CK (AE1/AE3), CK (AE1), CK7 (OV-TL12/30), CK8 (C51), CK18
(Dc-10), CK19 (RCK108), and EMA (Mc-5) showed strong expression, CEA
(COL-1), CK20 (Ks20. 4), and MUC-5AC (CLH2) showed moderate
expression, and MUC-2 (B306. 1) showed weak expression; CK17 (E3)
showed focal expression. CONCLUSIONS: The clinical manifestation,
macropathologic type, histological characteristics of gallbladder
adenocarcinoma with stromal fibrosis are different from other types of
adenocarcinoma. Its genesis may be related to chronic cholecystitis:
long-term inflammation causes regional hyperplasia and heterogeneity
of the gland body, lead to focal or regional thickening of the
gallbladder wall, and result in gallbladder adenocarcinoma with
stromal fibrosis finally.
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